Specific activity was 60,000 cpm/lig RNA Doublestranded Plabeled poliovirus RNA (RFRNA) (specific activity, 30,000 cpm/pAg RNA)wasprepared fromvirusinfected cells, and the purity was established as described (15) Doublestranded RNA labeled with 8Huridine (specific activity, 4000 cpm/,ug) extracted from purified reovirus type 3 with The reovirus core has also interested Harrison's lab because it may bear similarities to other doublestranded RNA viruses that are important pathogens In upcoming studies, Harrison's group will be looking at the relationship between the structure and function of the outer shell proteins that get reoviruses and rotaviruses into cells1 nm = 10 9 metre) across, have two icosahedral capsids, and contain a core of segmented, doublestranded RNA
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Double stranded rna is found in reovirus-A gradual release of doublestranded RNA occurred after the spreading of reovirus particles on urea in the cold Electron microscopy of virusprotein monolayers showed that each virion expelled about 11 fragments of μ, maximum total length ThisOSTIGOV Journal Article Distinct binding sites for zinc and doublestranded RNA in the reovirus outer capsid protein sigma3 Distinct binding sites for zinc and doublestranded RNA in the reovirus outer capsid protein sigma3 Full Record;
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Reovirus doublestranded RNA genomes and polyIC induce downregulation of hypoxiainducible factor 1α Author Hotani, Takuma, Tachibana, Masashi, Mizuguchi, Hiroyuki, Sakurai, Fuminori Source Biochemical and biophysical research communications 15 v460 pp ISSN X SubjectRe·o·vir·i·dae A family of doublestranded RNA viruses, some of which (Reovirus) previously were included with Echoviruses, and others (Orbivirus), with arboviruses Virions are 6080 nm in diameter, usually naked, and ether resistant;Those with a genome made of several pieces of doublestranded RNA Example reovirus Retroviruses Their RNA (also singlestranded) is copied by reverse transcriptase into a
This RNA is less dense than viral RNA and resists digestion by ribonuclease It is therefore, considered to be a doublestrandedform of poliovirus RNAAsignificant amount of this material is found in association with a membranous virusspecific particulate The recent demonstration of the natural occurrence of doublestranded RNA in reovirusThe reovirus mRNA in turn serves as a template for the synthesis of the complementary () strand The latter remains associated with the template, thus forming doublestranded virion RNA The virion associated enzymes can be activated in vitro to synthesize reovirus mRNAs with an m 7 GNine isolates of avian reovirus (ARV) from both healthy birds and birds with different clinical illness and one commercially available vaccine strain were selected and characterized by analysis of the migration pattern of their genomic doublestranded RNA (dsRNA) segments following separation by polyacrylamide gel electrophoresis Different electropherotypes were observed
RIGI contributes to IFN production by cells infected with reovirus or transfected with the doublestranded (ds)RNA segments of the reovirus The doublestranded RNA molecules are classified by size into three large (L), three medium (M), and four small (S) gene segments There are three reovirus serotypes, which can be differentiated by the capacity of antireovirus sera to neutralize viral infectivity and inhibit hemagglutination ( 29 , 33 )Reovirus re´ovi″rus any member of a family of etherresistant RNA viruses isolated from healthy children, children with febrile and afebrile upper respiratory disease, children with diarrhea, and many animals
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R (espiratory)e (nteric)o (rphan)virusThey stimulate the cellular upUPTAKE OF REOVIRUS DOUBLESTRANDED RNA IN MACROPHAGES IN VITRO ROLF SELJELID, SAMUEL C SILVERSTEIN, and ZANVIL A COHN INTRODUCTION Polycations have been reported to enhance the infectivity of single and doublestranded viral RNA (1), of doublestranded DNA (2), and of RNA tumor viruses (3);
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OSTIGOV Journal Article Crystallization of the avian reovirus doublestranded RNAbinding and core protein σA Crystallization of the avian reovirus doublestranded RNAbinding and core protein σA Full Record;By atomic absorption analysis, we determined that the reovirus outer capsid protein sigma 3, which binds doublestranded RNA (dsRNA), is a zinc metalloprotein Using Northwestern blots and a novel zinc blotting technique, we localized the zinc andIn brief, singlestranded (plusstranded, ss) and/or doublestranded (ds) RNA of reovirus serotype 3 (ST3 virus) is lipofected into L929 mouse fibroblasts together with a rabbit reticulocyte lysate in which ss or melted dsRNA has been translated After 8 hr the cells are then infected with a helper virus, ST2 reovirus
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The doublestranded (ds)RNA viruses represent a diverse group of viruses that vary widely in host range (humans, animals, plants, fungi, and bacteria), genome segment number (one to twelve), and virion organization (Tnumber, capsid layers, or turrets)By atomic absorption analysis, we determined that the reovirus outer capsid protein sigma 3, which binds doublestranded RNA (dsRNA), is a zinc metalloprotein Using Northwestern blots and a novel zinc blotting technique, we localized the zinc and dsRNAbinding activities of sigma 3 to distinct V8 proteasegenerated fragmentsThe virions of reoviruses (the name is a shortening of respiratory enteric orphan viruses) lack an outer envelope, appear spheroidal, measure about 70 nanometres (nm;
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Reoviruses are known to contain doublestranded (DS) RNA1 It was therefore surprising to find that RNA extracted from highly purified reovirus type 3 and analyzed without further fractionation had a purine/pyrimidine ratio of 15 and an adenylic acid content of more than 40 moles per cent In an effort to understand the basis for this The interaction between the helicase DHX33 and IPS1 as a novel pathway to sense doublestranded RNA and RNA viruses in myeloid dendritic cells Cell Mol Immunol 11, 49–57 (14) https//doiAbstract The distribution of label into newly synthesized doublestranded (ds) RNA in replicase particles isolated from reovirusinfected cells at 75 hr postinfection was determined A 30min exposure of infected cells to 3 Huridine labeled the RNA in both () and (−) strands These results indicate that any singlestranded (ss) RNA that is made at later times postinfection and that
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Although polyinosinicpolycytidylic acid (poly IC), an artificial doublestranded (ds) RNA, was found to be a ligand for MDA5 (26, 28), the motif recognized by MDA5 should be characterized, Reovirus genome RNA consists of 10 segments in three distinct classes called L, M, and S corresponding to their sizeThe S2 gene nucleotide sequences of prototype strains of the three reovirus serotypes Characterization of reovirus core protein σ2 We have also performed experiments to further characterize the doublestranded RNAbinding activity of σ2 and found that the capacity to bind doublestranded RNA is a property of the σ2 protein of prototype This is also called positivestranded RNA Examples poliovirus;
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A likely candidate for the role of such molecules is double stranded RNA (dsRNA) dsRNA is involved in numerous biological processes and at least three different pathways can respond to dsRNA in mammals, including sequenceindependent interferon response, editing by adenosine deaminases, and sequencespecific RNA interference (RNAi) (Chalupnikova et al,Doublestranded (ds) RNA viruses are a diverse group of viruses that vary widely in host range (humans, animals, plants, fungi, and bacteria), genome segment number (one to twelve), and virion organization (Tnumber, capsid layers, or turrets) Members of this group include the rotaviruses, renowned globally as the commonest cause of gastroenteritis in young children, and bluetongueA series of 94 isolates of reovirus from humans, cattle, and mice, showed extensive variability in the patterns of migration of the ten doublestranded RNA genome segments This variation was found in all three serotypes, and involved all ten genome segments, including the segment responsible for serological specificity
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Genomes contain doublestranded, segmented RNA (MW 1016 × 10 6 ); The annealed products migrated in acrylamide gels as doublestranded RNA, indicating efficient in vitro transcription Subviral particles containing reovirus RNA transcriptase have been isolated from extracts of virusinfected mouse fibroblast cellsCapsids are of icosohedral symmetry
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Differential Delivery of Genomic DoubleStranded RNA Causes Reovirus StrainSpecific Differences in Interferon Regulatory Factor 3 Activation Stuart JD (1) (2), Holm GH (3), Boehme KW (4) (2) Author information (1)Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USARe•o•vi•rus (ˌri oʊˈvaɪ rəs, ˈri oʊˌvaɪ) n, pl rus•es any of several large viruses of the family Reoviridae having doublestranded RNA and a polyhedral capsid includes viruses that cause gastroenteritis 1955–60;Reovirus a group of RNA viruses isolated from man, mammals, and birds The virus particle is 6090 nanometers in diameter The outer membrane (capsid), which protects the inner capsid with doublestranded ribonucleic acid, consists of protein subunits (capsomers) Reoviruses contain the enzyme RNAdependent RNA polymerase
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HHMI researchers have solved the structure of an important component of the reovirus, a doublestranded RNA virus that bears similarity to pathogens such as rotavirus, a potentially deadly cause The orthoreoviruses are infectious agents that possess a genome comprised of 10 doublestranded RNA segments encased in two concentric protein capsids Like virtually all RNA viruses, an RNAdependent RNA polymerase (RdRp) enzyme is required for viral propagation RdRp sequences have been determined for the prototype mammalian orthoreoviruses and for several other closelyrelated reovirusesRNA's (mRNA's) No doublestranded RNA has been found free within virusinfected cells other than that in newly sylnthesized virus and subviral particles The virus and subviral particles appear to be associated with virussynthesizing factories MaterialsVirus Dearing strain of reovirus 3, cloned,1 was used as stock virus after two passages
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Reovirus definition, any large virus of the family Reoviridae, having doublestranded RNA and a polyhedral capsid, including those causing infantile gastroenteritis See moreThe peculiarity of reovirus was underscored by the demonstration later that an RNA polymerase in the virion of reovirus is able to a symmetrically transcribe the doublestranded RNA (26) Th is was the first virionbound RNAdependent RNA polymerase ever foundRNA interference (RNAi) is a genetic regulatory system that functions to silence the activity of specific genes RNAi occurs naturally, through the production of nuclearencoded premicroRNA (premiRNA), and can be induced experimentally, using short segments of synthetic doublestranded RNA (dsRNA)
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Structure Of Genomic Segments 1 To 3 Of The 9b21 Reovirus A Ethidium Download Scientific Diagram
Differential Delivery of Genomic DoubleStranded RNA Causes Reovirus StrainSpecific Differences in Interferon Regulatory Factor 3 Activation Serotype 3 (T3) reoviruses induce substantially more type 1 interferon (IFNI) secretion than serotype 1 (T1) strains However, the mechanisms underlying differences in IFNI production between T1 and T3 reoviruses remain The genes of the Dearing strain of reovirus serotype 3, which consist of doublestranded RNA, have been cloned into pBR322 by tailing both strands of each gene with poly(A), transcribing them with reverse transcriptase, selfhybridizing the cognate plus and minus cDNA strands, incubating them with Escherichia coli DNA polymerase I to ensure that they are complete, and cloning the doublestrandedThe virions of doublestranded RNA bacteriophages of the family Cystoviridae possess a triplelayered structure surrounding the trisegmented genome Genomecontaining doubleshelled nucleocapsids are formed by a selfassembly process that can be accomplished in vitro using purified proteins and RNA constituents
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Parallels Among Positive Strand Rna Viruses Reverse Transcribing Viruses And Double Stranded Rna Viruses Nature Reviews Microbiology
Definition of reovirus any of a family (Reoviridae) of doublestranded RNA viruses that have a virion with icosahedral structural symmetry but may appear spherical, that have a capsid with one to three concentric protein layers, and that include many plant or animal pathogens (such as the rotaviruses and the causative agent of bluetongue)
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